32QA

Cryo-EM structure of Saccharomyces cerevisiae Erv14-Qdr2 complex in lipid nanodiscs (double Erv14 assembly)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	ER-derived vesicles protein ERV14	polymer	146	16940.9	2	UniProt (P53173) Pfam (PF03311)	Saccharomyces cerevisiae (brewer's yeast)
2	B (B)	Quinidine resistance protein 2	polymer	549	60527.6	1	UniProt (P40474) Pfam (PF07690)	Saccharomyces cerevisiae (brewer's yeast)
3	D, E, F, G, I... (A, B, C)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	9	Chemie (Y01) PubChem (65082)
4	H, N, O, P (B)	ERGOSTEROL	non-polymer		396.6	4	Chemie (ERG) PubChem (444679)
5	L (B)	(2R)-2-hydroxy-3-(phosphonooxy)propyl (9E)-octadec-9-enoate	non-polymer		436.5	1	Chemie (NKP) PubChem (49837858)

Sequence modifications

A, C: 1 - 138 (UniProt: P53173)

PDB	External Database	Details
Asp 139	-	expression tag
Tyr 140	-	expression tag
Lys 141	-	expression tag
Asp 142	-	expression tag
Asp 143	-	expression tag
Asp 144	-	expression tag
Asp 145	-	expression tag
Lys 146	-	expression tag

B: 1 - 542 (UniProt: P40474)

PDB	External Database	Details
Gly 543	-	expression tag
Glu 544	-	expression tag
Asn 545	-	expression tag
Leu 546	-	expression tag
Tyr 547	-	expression tag
Phe 548	-	expression tag
Gln 549	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	94409.4
Non-Polymers*	Number of molecules	14
	Total formula weight	6403.6
All*	Total formula weight	100813.0

*Water molecules are not included.