31OR
CryoEM structure of substrate engaged p97 in complex with Ufd1/Npl4 and C-terminal FAF1- State IV
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, E, J, N... (J, K, I, M, N...) | Polyubiquitin-B | polymer | 76 | 8576.8 | 6 | UniProt (P0CG47) | Homo sapiens (human) | |
| 2 | C (G) | Nuclear protein localization protein 4 homolog | polymer | 608 | 68202.0 | 1 | UniProt (Q8TAT6) | Homo sapiens (human) | Protein NPL4 |
| 3 | D (H) | Ubiquitin recognition factor in ER-associated degradation protein 1 | polymer | 307 | 34544.3 | 1 | UniProt (Q92890) | Homo sapiens (human) | Ubiquitin fusion degradation protein 1,UB fusion protein 1 |
| 4 | F, G, H, I, L... (E, B, A, F, C...) | Transitional endoplasmic reticulum ATPase | polymer | 806 | 89493.9 | 6 | UniProt (P55072) | Homo sapiens (human) | TER ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein,VCP |
| 5 | K (T) | FAS-associated factor 1 | polymer | 170 | 20227.7 | 1 | UniProt (Q9UNN5) | Homo sapiens (human) | hFAF1,UBX domain-containing protein 12,UBX domain-containing protein 3A |
| 6 | DA, GA, JA, LA, NA... (A, F, C, E, D...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 12 | Chemie (ADP) | |||
| 7 | BA, CA, EA, IA, OA... (A, F, C, E, B) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 10 | Chemie (BEF) | |||
| 8 | AA, FA, HA, KA, MA... (B, A, F, C, E) | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) PubChem (888) |
Sequence modifications
E, B, A, F, C, D: 1 - 806 (UniProt: P55072)
| PDB | External Database | Details |
|---|---|---|
| Glu 232 | Ala 232 | engineered mutation |
| Gln 578 | Glu 578 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 711398.1 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 6029.5 | |
| All* | Total formula weight | 717427.6 |
