31ON
CryoEM structure of substrate engaged p97 in complex with Ufd1/Npl4 and C-terminal FAF1-State I
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Transitional endoplasmic reticulum ATPase | polymer | 826 | 91665.2 | 6 | UniProt (P55072) | Homo sapiens (human) | TER ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein,VCP |
| 2 | G (G) | Nuclear protein localization protein 4 homolog | polymer | 608 | 68202.0 | 1 | UniProt (Q8TAT6) | Homo sapiens (human) | Protein NPL4 |
| 3 | H, I, J, K, L... (I, J, K, N, L...) | Polyubiquitin-B | polymer | 76 | 8576.8 | 6 | UniProt (P0CG47) | Homo sapiens (human) | |
| 4 | N (T) | FAS-associated factor 1 | polymer | 170 | 20227.7 | 1 | UniProt (Q9UNN5) | Homo sapiens (human) | hFAF1,UBX domain-containing protein 12,UBX domain-containing protein 3A |
| 5 | O (H) | Ubiquitin recognition factor in ER-associated degradation protein 1 | polymer | 307 | 34544.3 | 1 | UniProt (Q92890) | Homo sapiens (human) | Ubiquitin fusion degradation protein 1,UB fusion protein 1 |
| 6 | BA, DA, HA, JA, NA... (C, D, E, A, F...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 12 | Chemie (ADP) | |||
| 7 | AA, CA, GA, IA, KA... (C, D, E, A, B) | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) PubChem (888) | |||
| 8 | EA, FA, LA, MA, OA... (D, E, B, C) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 10 | Chemie (BEF) |
Sequence modifications
A, B, C, D, E, F: 1 - 806 (UniProt: P55072)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Glu 232 | Ala 232 | variant |
| Gln 578 | Glu 578 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 724426.4 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 6029.5 | |
| All* | Total formula weight | 730455.9 |
