31GL
Prescottella amidase, S181A mutant, diethyl toluene-2,4-dicarbamate soak
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | amidase | polymer | 475 | 50901.9 | 2 | UniProt (A0ABW9FQ31) | Prescottella equi | |
| 2 | C, G (A, B) | FORMIC ACID | non-polymer | 46.0 | 2 | Chemie (FMT) PubChem (284) | |||
| 3 | D (A) | Diethyl toluene-2,4-dicarbamate | non-polymer | 266.3 | 1 | Chemie (A1J91) | |||
| 4 | E, H (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) PubChem (888) | |||
| 5 | F, I (A, B) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 2 | Chemie (DMS) PubChem (679) | |||
| 6 | J, K (A, B) | water | water | 18.0 | 1367 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 472 (UniProt: A0ABW9FQ31)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Gly 37 | Asp 37 | conflict |
| Ser 153 | Thr 153 | conflict |
| Ala 181 | Ser 181 | engineered mutation |
| Met 293 | Leu 293 | conflict |
| Asp 303 | Glu 303 | conflict |
| Val 452 | Ala 452 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 101803.8 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 563.2 | |
| All* | Total formula weight | 102367.0 |
