31FW

Human tRNA ligase complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ashwin	polymer	259	28940.8	1	UniProt (Q9BVC5)	Homo sapiens (human)
2	B (B)	Protein FAM98B	polymer	330	37153.7	1	UniProt (Q52LJ0)	Homo sapiens (human)
3	C (C)	RNA transcription, translation and transport factor protein	polymer	244	28110.1	1	UniProt (Q9Y224)	Homo sapiens (human)	CLE7 homolog,CLE,hCLE
4	D (D)	ATP-dependent RNA helicase DDX1,Green fluorescent protein	polymer	329	36400.8	1	UniProt (Q92499) UniProt (P42212)	Homo sapiens (human)	DEAD box protein 1,DEAD box protein retinoblastoma,DBP-RB
5	E (R)	RNA-splicing ligase RtcB homolog	polymer	521	57142.2	1	UniProt (Q9Y3I0)	Homo sapiens (human)	3'-phosphate/5'-hydroxy nucleic acid ligase
6	F (R)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)

Sequence modifications

A: 1 - 232 (UniProt: Q9BVC5)

PDB	External Database	Details
Met -26	-	initiating methionine
Trp -25	-	expression tag
Ser -24	-	expression tag
His -23	-	expression tag
Pro -22	-	expression tag
Gln -21	-	expression tag
Phe -20	-	expression tag
Glu -19	-	expression tag
Lys -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Gly -15	-	expression tag
Trp -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Pro -11	-	expression tag
Gln -10	-	expression tag
Phe -9	-	expression tag
Glu -8	-	expression tag
Lys -7	-	expression tag
Pro -6	-	expression tag
Pro -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

D: 697 - 740 (UniProt: Q92499)

PDB	External Database	Details
Met 696	-	initiating methionine
Ser 741	-	linker
Phe 742	-	linker
Gly 743	-	linker
Ser 744	-	linker
Gly 745	-	linker
Ala 746	-	linker
Thr 747	-	linker
Asn 748	-	linker
Phe 749	-	linker
Ser 750	-	linker
Leu 751	-	linker
Leu 752	-	linker
Lys 753	-	linker
Gln 754	-	linker
Ala 755	-	linker
Gly 756	-	linker
Asp 757	-	linker
Val 758	-	linker
Glu 759	-	linker
Glu 760	-	linker
Asn 761	-	linker
Pro 762	-	linker
Gly 763	-	linker
Pro 764	-	linker
Gly 765	-	linker
Ser 766	-	linker
Gly 767	-	linker
Glu 768	-	linker
Gly 769	-	linker
Arg 770	-	linker
Gly 771	-	linker
Ser 772	-	linker
Leu 773	-	linker
Leu 774	-	linker
Thr 775	-	linker
Cys 776	-	linker
Gly 777	-	linker
Asp 778	-	linker
Val 779	-	linker
Glu 780	-	linker
Glu 781	-	linker
Asn 782	-	linker
Pro 783	-	linker
Gly 784	-	linker
Pro 785	-	linker
Met 786	-	linker
Val 787	-	linker

D: 788 - 1024 (UniProt: P42212)

PDB	External Database	Details
Leu 850	Phe 64	conflict
Thr 851	Ser 65	conflict
His 943	Gln 157	conflict
Lys 992	Ala 206	conflict
Leu 1017	His 231	conflict

R: 1 - 505 (UniProt: Q9Y3I0)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Pro -6	-	expression tag
Pro -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	187747.6
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	187771.9

*Water molecules are not included.