31DC

Human formylglycine-generating enzyme FGE-C336S variant crystallized without elastase treatment, with peptide CTPSR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Formylglycine-generating enzyme	polymer	323	36112.9	1	UniProt (Q8NBK3)	Homo sapiens (human)	FGE,C-alpha-formylglycine-generating enzyme 1,Sulfatase-modifying factor 1
2	B (P)	CYS-THR-PRO-SER-ARG	polymer	5	563.6	1		Homo sapiens
3	C (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	D, E (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA) PubChem (271)
5	F, G (A, P)	water	water		18.0	79	Chemie (HOH)

Sequence modifications

A: 73 - 374 (UniProt: Q8NBK3)

PDB	External Database	Details
Ala 64	-	expression tag
Asp 65	-	expression tag
Leu 66	-	expression tag
Gly 67	-	expression tag
Ser 68	-	expression tag
Ser 69	-	expression tag
Met 70	-	expression tag
Glu 71	-	expression tag
Phe 72	-	expression tag
Ser 336	Cys 336	engineered mutation
Ser 375	-	expression tag
Gly 376	-	expression tag
Arg 377	-	expression tag
Gly 378	-	expression tag
Ser 379	-	expression tag
His 380	-	expression tag
His 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	36676.5
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	37181.1

*Water molecules are not included.