31BG

Cryo-EM structure of Dopamine 3 receptor:Go complex bound to bitopic AB13-46A

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	354	40097.4	1	UniProt (P09471)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	355	39374.0	1	UniProt (P54311)	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (R)	Green fluorescent protein,D(3) dopamine receptor	polymer	669	74660.9	1	UniProt (P42212) UniProt (P35462)	Aequorea victoria	Dopamine D3 receptor
5	E (R)	~{N}-[2-[(1~{R},2~{S})-2-[[(2~{S},5~{S})-2-(6-azanylpyridin-3-yl)-5-methyl-morpholin-4-yl]methyl]cyclopropyl]ethyl]-6-methoxy-1~{H}-indole-2-carboxamide	non-polymer		463.6	1	Chemie (A1J83)

Sequence modifications

A: 1 - 354 (UniProt: P09471)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Glu 54	Lys 54	engineered mutation
Ala 204	Gly 204	engineered mutation
Ala 246	Glu 246	engineered mutation
Lys 249	Met 249	engineered mutation
Ser 326	Ala 326	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -14	-	initiating methionine
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

R: -243 - -7 (UniProt: P42212)

PDB	External Database	Details
Met -268	-	initiating methionine
Lys -267	-	expression tag
Thr -266	-	expression tag
Ile -265	-	expression tag
Ile -264	-	expression tag
Ala -263	-	expression tag
Leu -262	-	expression tag
Ser -261	-	expression tag
Tyr -260	-	expression tag
Ile -259	-	expression tag
Phe -258	-	expression tag
Cys -257	-	expression tag
Leu -256	-	expression tag
Val -255	-	expression tag
Phe -254	-	expression tag
Ala -253	-	expression tag
Asp -252	-	expression tag
Tyr -251	-	expression tag
Lys -250	-	expression tag
Asp -249	-	expression tag
Asp -248	-	expression tag
Asp -247	-	expression tag
Asp -246	-	expression tag
Lys -245	-	expression tag
Val -244	-	expression tag
Leu -181	Phe 64	conflict
Thr -180	Ser 65	conflict
Leu -14	His 231	conflict
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker

R: 2 - 400 (UniProt: P35462)

PDB	External Database	Details
Ser 9	Gly 9	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	161993.5
Non-Polymers*	Number of molecules	1
	Total formula weight	463.6
All*	Total formula weight	162457.0

*Water molecules are not included.