30WC

Structure of E. coli WbaP in complex with a megabody in the presence of UDP-Gal processed in C2 symmetry

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Undecaprenyl-phosphate galactose phosphotransferase WbaP	polymer	496	57450.4	2	UniProt (A0A6D1CLM9)	Escherichia coli
2	C, D (C, D)	Megabody	polymer	524	56575.0	2		Lama glama
3	E, F (A, B)	GALACTOSE-URIDINE-5'-DIPHOSPHATE	non-polymer		566.3	2	Chemie (GDU)

Sequence modifications

A, B: 1 - 476 (UniProt: A0A6D1CLM9)

PDB	External Database	Details
Arg 6	Lys 6	conflict
Val 8	Ile 8	conflict
Ser 9	Gly 9	conflict
Ser 11	Thr 11	conflict
Thr 13	Ala 13	conflict
Ala 15	Val 15	conflict
Val 17	Ser 17	conflict
Leu 22	Phe 22	conflict
Thr 23	Val 23	conflict
Leu 30	Val 30	conflict
Val 32	Leu 32	conflict
Ser 34	Ala 34	conflict
Leu 35	Val 35	conflict
Thr 36	Ser 36	conflict
Leu 37	Met 37	conflict
Glu 38	Thr 38	conflict
His 39	Asp 39	conflict
Gln 41	Glu 41	conflict
Gln 42	Thr 42	conflict
Gln 46	Gly 46	conflict
Asp 47	Glu 47	conflict
Ile 49	Leu 49	conflict
Glu 50	Asp 50	conflict
Gly 51	Thr 51	conflict
Gly 54	Leu 54	conflict
Trp 57	Leu 57	conflict
Phe 82	Tyr 82	conflict
Val 92	Ile 92	conflict
Ile 96	Val 96	conflict
Val 99	Met 99	conflict
Ile 101	Val 101	conflict
Ile 102	Met 102	conflict
Met 116	Leu 116	conflict
Val 119	Leu 119	conflict
Phe 120	Leu 120	conflict
Ala 122	Val 122	conflict
Ala 123	Phe 123	conflict
Leu 128	Ala 128	conflict
Thr 131	Gly 131	conflict
Ala 132	Thr 132	conflict
Tyr 134	Trp 134	conflict
Ile 135	Cys 135	conflict
Ser 138	Lys 138	conflict
Leu 139	Ile 139	conflict
Leu 141	Phe 141	conflict
Met 143	Leu 143	conflict
His 156	Ile 156	conflict
Asn 163	Tyr 163	conflict
Asn 166	Arg 166	conflict
Asn 171	Lys 171	conflict
Val 172	Ile 172	conflict
Val 176	Ile 176	conflict
Val 178	Ser 178	conflict
Asp 179	Gly 179	conflict
Asp 180	Ser 180	conflict
-	Ala 181	deletion
-	Ser 182	deletion
Asn 183	Thr 185	conflict
Gly 184	His 186	conflict
Ser 185	Gly 187	conflict
Lys 186	Thr 188	conflict
Asn 187	Asp 189	conflict
Thr 188	Val 190	conflict
Asp 190	Asn 192	conflict
Ser 191	Gly 193	conflict
Val 192	Ile 194	conflict
Gln 193	Glu 195	conflict
Ser 196	Lys 198	conflict
Val 197	Ala 199	conflict
Asp 198	Ser 200	conflict
Thr 200	Asp 202	conflict
Thr 203	Glu 205	conflict
Thr 204	Ser 206	conflict
Val 205	Ile 207	conflict
His 208	Arg 210	conflict
His 218	Thr 220	conflict
Gln 219	Glu 221	conflict
Ile 222	Thr 224	conflict
Arg 233	Lys 235	conflict
Met 247	Val 249	conflict
Gln 269	His 271	conflict
Ala 278	Ile 280	conflict
Leu 279	Ile 281	conflict
Ala 282	Leu 284	conflict
Ile 285	Val 287	conflict
Ser 290	Gly 292	conflict
Ile 293	Phe 295	conflict
Val 294	Ile 296	conflict
Val 298	Leu 300	conflict
Leu 300	Ile 302	conflict
Gln 304	Tyr 306	conflict
Ser 312	Pro 314	conflict
Gly 323	Asn 325	conflict
Arg 345	Asn 347	conflict
Glu 348	Ala 350	conflict
Asn 349	Thr 351	conflict
Pro 351	Asp 353	conflict
Lys 352	Ser 354	conflict
Lys 354	Arg 356	conflict
Ala 355	His 357	conflict
Asp 358	Glu 360	conflict
Ala 359	Met 361	conflict
Asp 365	Asn 367	conflict
Phe 375	Leu 377	conflict
Asp 416	Ala 418	conflict
Ile 421	Ser 423	conflict
Ile 466	Thr 468	conflict
Gly 467	Thr 469	conflict
Lys 472	Arg 474	conflict
Leu 477	-	expression tag
Glu 478	-	expression tag
Glu 479	-	expression tag
Asn 480	-	expression tag
Leu 481	-	expression tag
Tyr 482	-	expression tag
Phe 483	-	expression tag
Gln 484	-	expression tag
Gly 485	-	expression tag
Ala 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag
His 496	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	228050.9
Non-Polymers*	Number of molecules	2
	Total formula weight	1132.6
All*	Total formula weight	229183.5

*Water molecules are not included.