30MN

PLD-fold vaccinia virus endonuclease K4 with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Virion nicking-joining enzyme	polymer	450	52079.2	4	UniProt (P20537)	Orthopoxvirus vaccinia	Phospholipase-D-like protein K4
2	E, F (Y, Z)	19mer-ssDNA-primer	polymer	19	6005.9	2		synthetic construct

Sequence modifications

A, B, C, D: 2 - 424 (UniProt: P20537)

PDB	External Database	Details
Met -25	-	initiating methionine
Ser -24	-	expression tag
Tyr -23	-	expression tag
Tyr -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Ala 1	-	expression tag
Gln 115	His 115	engineered mutation
Gln 325	His 325	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	220328.7
All*	Total formula weight	220328.7

*Water molecules are not included.