30GA

Cryo-EM structure of the PseCascade-TniQ-TnsC-TnsAB holocomplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (1)	CRISPR RNA	polymer	93	30196.0	1		Pseudoalteromonas sp. S983
2	B, E (2, 5)	TS_RE-transferred	polymer	145	44743.5	2		Pseudoalteromonas sp. S983
3	C, G (3, 7)	Non-target DNA strand	polymer	90	27669.7	2		Pseudoalteromonas sp. S983
4	D, F (4, 6)	RE_non-trasferred	polymer	54	16520.6	2		Pseudoalteromonas sp. S983
5	H, I, J, K, L... (A, B, C, D, E...)	Cas7.1	polymer	350	39845.1	6	UniProt (A0ABF7PQB9)	Pseudoalteromonas sp. S983
6	N (G)	Cas8	polymer	695	79290.2	1	UniProt (A0ABF7PQB8)	Pseudoalteromonas sp. S983
7	O (H)	Cas6	polymer	237	26631.1	1	UniProt (A0ABF7PQC0)	Pseudoalteromonas sp. S983
8	P, Q (I, J)	TniQ.1	polymer	432	49832.4	2	UniProt (A0ABF7PQC1)	Pseudoalteromonas sp. S983
9	R, S, T, U, V... (K, L, M, N, O...)	AAA+ ATPase domain-containing protein	polymer	333	38609.9	7	UniProt (A0A290S0Z4)	Pseudoalteromonas sp. S983
10	AA, BA, CA, DA, EA... (R, S, T, U, V...)	Maltose/maltodextrin-binding periplasmic protein,TnsA endonuclease N-terminal domain-containing protein,TnsB transposase	polymer	1258	142301.8	11	UniProt (P0AEX9)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
11	AB, BB, CB, JA, KA... (a, b, 2, 5, K...)	MAGNESIUM ION	non-polymer		24.3	13	Chemie (MG) PubChem (888)
12	LA, NA, PA, RA, TA... (K, L, M, N, O...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	7	Chemie (ATP)

Sequence modifications

H: 1 - 203 (UniProt: A0ABF7PQC0)

PDB	External Database	Details
Ser 204	-	expression tag
Ala 205	-	expression tag
Trp 206	-	expression tag
Ser 207	-	expression tag
His 208	-	expression tag
Pro 209	-	expression tag
Gln 210	-	expression tag
Phe 211	-	expression tag
Glu 212	-	expression tag
Lys 213	-	expression tag
Gly 214	-	expression tag
Gly 215	-	expression tag
Gly 216	-	expression tag
Ser 217	-	expression tag
Gly 218	-	expression tag
Gly 219	-	expression tag
Gly 220	-	expression tag
Ser 221	-	expression tag
Gly 222	-	expression tag
Gly 223	-	expression tag
Ser 224	-	expression tag
Ala 225	-	expression tag
Trp 226	-	expression tag
Ser 227	-	expression tag
His 228	-	expression tag
Pro 229	-	expression tag
Gln 230	-	expression tag
Phe 231	-	expression tag
Glu 232	-	expression tag
Lys 233	-	expression tag
Ser 234	-	expression tag
Gly 235	-	expression tag
Gly 236	-	expression tag
Gly 237	-	expression tag

I, J: 1 - 409 (UniProt: A0ABF7PQC1)

PDB	External Database	Details
Met -22	-	initiating methionine
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Gly -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

K, L, M, N, O, P, Q: 1 - 333 (UniProt: A0A290S0Z4)

PDB	External Database	Details
Lys 11	Gln 11	conflict
Thr 27	Ala 27	conflict
Cys 47	Gly 47	conflict
Val 84	Ile 84	conflict
Val 98	Ile 98	conflict
Ile 112	Met 112	conflict
Asp 120	Glu 120	conflict
Ala 219	Val 219	conflict
Lys 221	Gln 221	conflict
Val 226	Glu 226	conflict
Asn 234	His 234	conflict
Ile 237	Met 237	conflict
Leu 255	Met 255	conflict
Thr 275	Ala 275	conflict
Ala 276	Val 276	conflict
Asp 278	Glu 278	conflict
Glu 287	Asp 287	conflict
Lys 288	Ser 288	conflict
Ser 291	Leu 291	conflict
Glu 303	Ile 303	conflict
Ile 308	Thr 308	conflict
Asp 326	Gly 326	conflict
Val 328	Lys 328	conflict

R, S, T, U, V, W, X, a, b, c, d: -392 - -27 (UniProt: P0AEX9)

PDB	External Database	Details
Met -406	-	initiating methionine
Gly -405	-	expression tag
Ser -404	-	expression tag
Ser -403	-	expression tag
His -402	-	expression tag
His -401	-	expression tag
His -400	-	expression tag
His -399	-	expression tag
His -398	-	expression tag
His -397	-	expression tag
Gly -396	-	expression tag
Ser -395	-	expression tag
Ser -394	-	expression tag
Met -393	-	expression tag
Asn -26	-	linker
Ser -25	-	linker
Ser -24	-	linker
Ser -23	-	linker
Asn -22	-	linker
Asn -21	-	linker
Asn -20	-	linker
Asn -19	-	linker
Asn -18	-	linker
Asn -17	-	linker
Asn -16	-	linker
Asn -15	-	linker
Asn -14	-	linker
Asn -13	-	linker
Leu -12	-	linker
Gly -11	-	linker
Ile -10	-	linker
Glu -9	-	linker
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Ser -2	-	linker
Asn -1	-	linker
Ala 0	-	linker

R, S, T, U, V, W, X, a, b, c, d: 1 - 238 (PDB: 30GA)
R, S, T, U, V, W, X, a, b, c, d: 239 - 851 (PDB: 30GA)

Contents of the asymmetric unit

Polymers	Number of chains	35
	Total formula weight	2488310.2
Non-Polymers*	Number of molecules	20
	Total formula weight	3866.2
All*	Total formula weight	2492176.4

*Water molecules are not included.