30FF
TFIID-containing RNA polymerase II pre-initiation complex in the presence of the +1 nucleosome
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (0) | General transcription and DNA repair factor IIH helicase subunit XPB | polymer | 782 | 89404.7 | 1 | UniProt (P19447) | Homo sapiens (human) | TFIIH subunit XPB,Basic transcription factor 2 89 kDa subunit,BTF2 p89,DNA excision repair protein ERCC-3,DNA repair protein complementing XP-B cells,TFIIH basal transcription factor complex 89 kDa subunit,TFIIH p89,Xeroderma pigmentosum group B-complementing protein |
| 2 | B (1) | TFIIH basal transcription factor complex helicase XPD subunit | polymer | 760 | 87021.1 | 1 | UniProt (P18074) | Homo sapiens (human) | Basic transcription factor 2 80 kDa subunit,BTF2 p80,CXPD,DNA excision repair protein ERCC-2,DNA repair protein complementing XP-D cells,TFIIH basal transcription factor complex 80 kDa subunit,TFIIH p80,Xeroderma pigmentosum group D-complementing protein |
| 3 | C (2) | General transcription factor IIH subunit 1 | polymer | 548 | 62116.5 | 1 | UniProt (P32780) | Homo sapiens (human) | Basic transcription factor 2 62 kDa subunit,BTF2 p62,General transcription factor IIH polypeptide 1,TFIIH basal transcription factor complex p62 subunit |
| 4 | D (3) | General transcription factor IIH subunit 4 | polymer | 462 | 52245.2 | 1 | UniProt (Q92759) | Homo sapiens (human) | Basic transcription factor 2 52 kDa subunit,BTF2 p52,General transcription factor IIH polypeptide 4,TFIIH basal transcription factor complex p52 subunit |
| 5 | E (4) | General transcription factor IIH subunit 2 | polymer | 395 | 44482.0 | 1 | UniProt (Q13888) | Homo sapiens (human) | Basic transcription factor 2 44 kDa subunit,BTF2 p44,General transcription factor IIH polypeptide 2,TFIIH basal transcription factor complex p44 subunit |
| 6 | F (5) | General transcription factor IIH subunit 3 | polymer | 308 | 34416.0 | 1 | UniProt (Q13889) | Homo sapiens (human) | Basic transcription factor 2 34 kDa subunit,BTF2 p34,General transcription factor IIH polypeptide 3,TFIIH basal transcription factor complex p34 subunit |
| 7 | G (6) | General transcription factor IIH subunit 5 | polymer | 71 | 8060.4 | 1 | UniProt (Q6ZYL4) | Homo sapiens (human) | General transcription factor IIH polypeptide 5,TFB5 ortholog,TFIIH basal transcription factor complex TTD-A subunit |
| 8 | H (7) | CDK-activating kinase assembly factor MAT1 | polymer | 309 | 35874.0 | 1 | UniProt (P51948) | Homo sapiens (human) | CDK7/cyclin-H assembly factor,Cyclin-G1-interacting protein,Menage a trois,RING finger protein 66,RING finger protein MAT1,p35,p36 |
| 9 | I (8) | Cyclin-dependent kinase 7 | polymer | 346 | 39090.3 | 1 | UniProt (P50613) | Homo sapiens (human) | 39 kDa protein kinase,p39 Mo15,CDK-activating kinase 1,Cell division protein kinase 7,Serine/threonine-protein kinase 1,TFIIH basal transcription factor complex kinase subunit |
| 10 | J (9) | Cyclin-H | polymer | 323 | 37695.5 | 1 | UniProt (P51946) | Homo sapiens (human) | MO15-associated protein,p34,p37 |
| 11 | K (A) | DNA-directed RNA polymerase subunit | polymer | 1984 | 218889.5 | 1 | UniProt (A0A7M4DUC2) | Sus scrofa (pig) | |
| 12 | L (B) | DNA-directed RNA polymerase subunit beta | polymer | 1300 | 147938.6 | 1 | UniProt (I3LGP4) | Sus scrofa (pig) | |
| 13 | M (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) | Sus scrofa (pig) | |
| 14 | N (D) | DNA-directed RNA polymerase II subunit RPB4 | polymer | 184 | 20962.6 | 1 | UniProt (A0A287ADR4) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit D,DNA-directed RNA polymerase II subunit rpb4 |
| 15 | O (DA) | Transcription initiation factor TFIID subunit 1 | polymer | 1893 | 214992.5 | 1 | UniProt (P21675) | Homo sapiens (human) | Cell cycle gene 1 protein,TBP-associated factor 250 kDa,p250,Transcription initiation factor TFIID 250 kDa subunit,TAF(II)250,TAFII-250,TAFII250 |
| 16 | P (DB) | Transcription initiation factor TFIID subunit 2 | polymer | 1199 | 137160.0 | 1 | UniProt (Q6P1X5) | Homo sapiens (human) | 150 kDa cofactor of initiator function,RNA polymerase II TBP-associated factor subunit B,TBP-associated factor 150 kDa,Transcription initiation factor TFIID 150 kDa subunit,TAF(II)150,TAFII-150,TAFII150 |
| 17 | Q, Z (DD, Dd) | Transcription initiation factor TFIID subunit 4 | polymer | 1085 | 110221.9 | 2 | UniProt (O00268) | Homo sapiens (human) | RNA polymerase II TBP-associated factor subunit C,TBP-associated factor 4,Transcription initiation factor TFIID 130 kDa subunit,TAF(II)130,TAFII-130,TAFII130,Transcription initiation factor TFIID 135 kDa subunit,TAF(II)135,TAFII-135,TAFII135 |
| 18 | AA, R (DE, De) | Transcription initiation factor TFIID subunit 5 | polymer | 800 | 86932.1 | 2 | UniProt (Q8C092) | Homo sapiens (human) | Transcription initiation factor TFIID 100 kDa subunit,TAF(II)100,TAFII-100,TAFII100 |
| 19 | BA, S (DF, Df) | Transcription initiation factor TFIID subunit 6 | polymer | 677 | 72749.3 | 2 | UniProt (P49848) | Homo sapiens (human) | RNA polymerase II TBP-associated factor subunit E,Transcription initiation factor TFIID 70 kDa subunit,TAF(II)70,TAFII-70,TAFII70,Transcription initiation factor TFIID 80 kDa subunit,TAF(II)80,TAFII-80,TAFII80 |
| 20 | T (DG) | Transcription initiation factor TFIID subunit 7 | polymer | 349 | 40325.1 | 1 | UniProt (Q4R5A5) | Homo sapiens (human) | |
| 21 | U (DH) | Transcription initiation factor TFIID subunit 8 | polymer | 310 | 34304.4 | 1 | UniProt (Q7Z7C8) | Homo sapiens (human) | Protein taube nuss,TBP-associated factor 43 kDa,TBP-associated factor 8,Transcription initiation factor TFIID 43 kDa subunit,TAFII-43,TAFII43,hTAFII43 |
| 22 | CA, V (DI, Di) | Transcription initiation factor TFIID subunit 9 | polymer | 264 | 29006.8 | 2 | UniProt (Q17QQ4) | Homo sapiens (human) | |
| 23 | DA, W (DJ, Dj) | Transcription initiation factor TFIID subunit 10 | polymer | 218 | 21731.2 | 2 | UniProt (Q12962) | Homo sapiens (human) | STAF28,Transcription initiation factor TFIID 30 kDa subunit,TAF(II)30,TAFII-30,TAFII30 |
| 24 | FA, X (DL, Dl) | Transcription initiation factor TFIID subunit 12 | polymer | 161 | 17948.5 | 2 | UniProt (Q16514) | Homo sapiens (human) | Transcription initiation factor TFIID 20/15 kDa subunits,TAFII-20/TAFII-15,TAFII20/TAFII15 |
| 25 | Y (Dc) | Transcription initiation factor TFIID subunit 3 | polymer | 929 | 103769.3 | 1 | Homo sapiens | ||
| 26 | EA (Dk) | Transcription initiation factor TFIID subunit 11 | polymer | 211 | 23340.1 | 1 | UniProt (Q15544) | Homo sapiens (human) | TFIID subunit p30-beta,Transcription initiation factor TFIID 28 kDa subunit,TAF(II)28,TAFII-28,TAFII28 |
| 27 | GA (Dm) | Transcription initiation factor TFIID subunit 13 | polymer | 124 | 14307.1 | 1 | UniProt (Q148M7) | Homo sapiens (human) | Transcription initiation factor TFIID 18 kDa subunit,TAF(II)18,TAFII-18,TAFII18 |
| 28 | HA (E) | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 210 | 24644.3 | 1 | UniProt (A0A4X1VTX4) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit E,RPB5 homolog |
| 29 | IA (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 30 | JA (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) | Sus scrofa (pig) | |
| 31 | KA (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) | Sus scrofa (pig) | |
| 32 | LA (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 33 | MA (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A8W4F9W9) | Sus scrofa (pig) | DNA-directed RNA polymerase III subunit L |
| 34 | NA (K) | RNA polymerase II subunit J | polymer | 117 | 13310.3 | 1 | UniProt (A0A8D0TE17) | Sus scrofa (pig) | |
| 35 | OA (L) | RNA polymerase II subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A8D0JYF1) | Sus scrofa (pig) | |
| 36 | PA (M) | Transcription initiation factor IIB | polymer | 316 | 34877.9 | 1 | UniProt (Q00403) | Homo sapiens (human) | General transcription factor TFIIB,S300-II |
| 37 | QA (N) | Non-template DNA | polymer | 227 | 70013.5 | 1 | unidentified adenovirus | ||
| 38 | RA (O) | TATA-box-binding protein | polymer | 339 | 37729.9 | 1 | UniProt (P20226) | Homo sapiens (human) | TATA sequence-binding protein,TATA-binding factor,TATA-box factor,Transcription initiation factor TFIID TBP subunit |
| 39 | SA (Q) | General transcription factor IIF subunit 1 | polymer | 517 | 58343.6 | 1 | UniProt (P35269) | Homo sapiens (human) | General transcription factor IIF 74 kDa subunit,Transcription initiation factor IIF subunit alpha,TFIIF-alpha,Transcription initiation factor RAP74 |
| 40 | TA (R) | General transcription factor IIF subunit 2 | polymer | 249 | 28427.3 | 1 | UniProt (P13984) | Homo sapiens (human) | ATP-dependent helicase GTF2F2,General transcription factor IIF 30 kDa subunit,Transcription initiation factor IIF subunit beta,TFIIF-beta,Transcription initiation factor RAP30 |
| 41 | UA (T) | Template DNA | polymer | 227 | 70177.6 | 1 | unidentified adenovirus | ||
| 42 | VA (U) | Transcription initiation factor IIA subunit 1 | polymer | 376 | 41544.6 | 1 | UniProt (P52655) | Homo sapiens (human) | General transcription factor IIA subunit 1,TFIIAL,Transcription initiation factor TFIIA 42 kDa subunit,TFIIA-42 |
| 43 | WA (V) | Transcription initiation factor IIA subunit 2 | polymer | 109 | 12469.1 | 1 | UniProt (P52657) | Homo sapiens (human) | General transcription factor IIA subunit 2,TFIIA p12 subunit,TFIIA-12,TFIIAS,Transcription initiation factor IIA gamma chain,TFIIA-gamma |
| 44 | XA (W) | General transcription factor IIE subunit 1 | polymer | 439 | 49516.1 | 1 | UniProt (P29083) | Homo sapiens (human) | General transcription factor IIE 56 kDa subunit,Transcription initiation factor IIE subunit alpha,TFIIE-alpha |
| 45 | YA (X) | Transcription initiation factor IIE subunit beta | polymer | 291 | 33106.8 | 1 | UniProt (P29084) | Homo sapiens (human) | TFIIE-beta,General transcription factor IIE subunit 2 |
| 46 | DB, ZA (a, e) | Histone H3.2 | polymer | 136 | 15421.1 | 2 | UniProt (P84233) | Xenopus laevis (African clawed frog) | |
| 47 | AB, EB (b, f) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) | Xenopus laevis (African clawed frog) | |
| 48 | BB, FB (c, g) | Histone H2A type 1 | polymer | 130 | 14093.4 | 2 | UniProt (P06897) | Xenopus laevis (African clawed frog) | |
| 49 | CB, GB (d, h) | Histone H2B 1.1 | polymer | 126 | 13965.3 | 2 | UniProt (P02281) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 50 | AC, BC, HB, IB, JB... (2, 0, N) | (2S)-2-aminopropan-1-ol | non-polymer | 75.1 | 24 | Chemie (2A1) PubChem (80307) | |||
| 51 | CC, OB, YB (2, 0) | ALANINE | non-polymer | 89.1 | 3 | Chemie (ALA) PubChem (5950) | |||
| 52 | WB (1) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 53 | AD, DC, EC, FC, GC... (W, 4, 5, 7, A...) | ZINC ION | non-polymer | 65.4 | 17 | Chemie (ZN) PubChem (32051) | |||
| 54 | KC (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) |
Sequence modifications
DE, De: 1 - 800 (UniProt: Q8C092)
DI, Di: 1 - 264 (UniProt: Q17QQ4)
c, g: 1 - 130 (UniProt: P06897)
| PDB | External Database | Details |
|---|---|---|
| Thr 40 | Pro 40 | conflict |
| - | Gly 50 | deletion |
| Ala 53 | Val 54 | conflict |
| Ser 54 | Ala 55 | conflict |
| Ser 55 | Ala 56 | conflict |
| Ser 56 | Ala 57 | conflict |
| Thr 57 | Ala 58 | conflict |
| Thr 67 | Gly 68 | conflict |
| Ala 85 | Pro 86 | conflict |
| Pro 86 | Ala 87 | conflict |
| Asp 87 | Glu 88 | conflict |
| Lys 108 | Asn 109 | conflict |
| Gly 121 | Arg 122 | conflict |
| Val 136 | Leu 137 | conflict |
| Ser 138 | Gly 139 | conflict |
| Val 143 | Ala 144 | conflict |
| Thr 144 | Ala 145 | conflict |
| Ala 155 | Val 156 | conflict |
| Pro 158 | Ser 159 | conflict |
| Asp 162 | Glu 163 | conflict |
| Gly 170 | Val 171 | conflict |
| Ala 171 | Thr 172 | conflict |
| Thr 172 | Ser 173 | conflict |
| Val 174 | Phe 175 | conflict |
| Gly 188 | Ala 189 | conflict |
| Ser 310 | Tyr 311 | conflict |
| Phe 635 | Tyr 636 | conflict |
| Ile 741 | Val 742 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Thr 6 | Met 6 | conflict |
| Thr 138 | Ala 138 | conflict |
| Gln 168 | Pro 168 | conflict |
| Thr 169 | Ala 169 | conflict |
| Met 180 | Val 180 | conflict |
| Met 231 | Val 231 | conflict |
| Ser 240 | Ala 240 | conflict |
| Arg 248 | His 248 | conflict |
| Glu 249 | Asp 249 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 100 | Gly 100 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 59 |
| Total formula weight | 2819091.2 | |
| Non-Polymers* | Number of molecules | 46 |
| Total formula weight | 3557.8 | |
| All* | Total formula weight | 2822649.0 |
