30CW

Class 3 L-asparaginase from Colletotrichum fructicola with active site Ser51 modified with boric acid - glycerol ester

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	L-asparaginase ii	polymer	363	38968.2	2	UniProt (L2FY35)	Colletotrichum fructicola
2	B, C (B, C)	L-asparaginase ii	polymer	363	39029.0	2	UniProt (L2FY35)	Colletotrichum fructicola
3	E, I, M (A, C, D)	GLYCINE	non-polymer		75.1	3	Chemie (GLY) PubChem (750)
4	F, H, L, N (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN) PubChem (32051)
5	G, O (A, D)	(S)-(2,3-DIHYDROXYPROPOXY)TRIHYDROXYBORATE	non-polymer		152.9	2	Chemie (2DB) PubChem (11957419)
6	J, K (C)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
7	P, Q, R, S (A, B, C, D)	water	water		18.0	299	Chemie (HOH)

Sequence modifications

A, D: 1 - 357 (UniProt: L2FY35)

PDB	External Database	Details
Gly -5	-	expression tag
Ile -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

B, C: 1 - 357 (UniProt: L2FY35)

PDB	External Database	Details
Gly -5	-	expression tag
Ile -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	155994.3
Non-Polymers*	Number of molecules	11
	Total formula weight	916.8
All*	Total formula weight	156911.1

*Water molecules are not included.