30AD

Complex between IgE-Fc and anti-IgE Fab aeFab81

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Isoform 1 of Immunoglobulin heavy constant epsilon	polymer	327	36354.8	2	UniProt (P01854) Pfam (PF07654)	Homo sapiens (human)	Ig epsilon chain C region,Ig epsilon chain C region ND
2	C (H)	aeFab81 heavy chain	polymer	216	22770.3	1		Oryctolagus cuniculus
3	D (L)	aeFab81 light chain	polymer	214	22709.1	1		Oryctolagus cuniculus
4	E (C)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G55220VL)
5	F (D)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G80966KZ)
6	G, H, K (A, B, H)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG) PubChem (8117)
7	I, L (H, L)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4) PubChem (8200)
8	J (H)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS) PubChem (3777159)

Sequence modifications

A, B: 224 - 547 (UniProt: P01854)

PDB	External Database	Details
Asp 222	-	expression tag
Ile 223	-	expression tag
Ala 225	Cys 105	engineered mutation
Gln 265	Asn 146	engineered mutation
Gln 371	Asn 252	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	118189.0
Branched	Number of molecules	2
	Total formula weight	2632.3
Non-Polymers*	Number of molecules	6
	Total formula weight	829.0
All*	Total formula weight	121650.3

*Water molecules are not included.