2ZZ3

Covalent complex of orotidine monophosphate decarboxylase D70A mutant from M. thermoautotrophicus with 6-cyano-UMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Orotidine 5'-phosphate decarboxylase	polymer	252	27335.4	2	UniProt (O26232) Pfam (PF00215) In PDB	Methanothermobacter thermautotrophicus	OMP DECARBOXYLASE, OMPDCASE, ODCase
2	A, B	6-cyanouridine 5'-phosphate	non-polymer		349.2	2	Chemie (6CN)
3	A, B	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4		water	water		18.0	239	Chemie (HOH)

Sequence modifications

A, B: 1 - 228 (UniProt: O26232)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 70	Asp 70	engineered mutation
Pro 101	Arg 101	SEE REMARK 999
Arg 226	Leu 226	engineered mutation
Ile 227	Asn 227	engineered mutation
Glu 229	-	expression tag
Asp 230	-	expression tag
Pro 231	-	expression tag
Ala 232	-	expression tag
Ala 233	-	expression tag
Asn 234	-	expression tag
Lys 235	-	expression tag
Ala 236	-	expression tag
Arg 237	-	expression tag
Lys 238	-	expression tag
Glu 239	-	expression tag
Ala 240	-	expression tag
Glu 241	-	expression tag
Leu 242	-	expression tag
Ala 243	-	expression tag
Ala 244	-	expression tag
Ala 245	-	expression tag
Thr 246	-	expression tag
Ala 247	-	expression tag
Glu 248	-	expression tag
Gln 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54670.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1066.8
All*	Total formula weight	55737.5

*Water molecules are not included.