2ZMA
Crystal Structure of 6-Aminohexanoate-dimer Hydrolase S112A/G181D/H266N/D370Y Mutant with Substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 6-aminohexanoate-dimer hydrolase | polymer | 392 | 42905.6 | 1 | UniProt (P07061) UniProt (P07062) Pfam (PF00144) | Flavobacterium sp. | Nylon oligomers-degrading enzyme EII, Nylon oligomers-degrading enzyme EII' |
| 2 | B, C, D, E, F (A) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 3 | G, H (A) | 6-AMINOHEXANOIC ACID | non-polymer | 131.2 | 2 | Chemie (ACA) | |||
| 4 | I (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 5 | J, K, L, M, N (A) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | O (A) | water | water | 18.0 | 430 | Chemie (HOH) |
Sequence modifications
A: 1 - 21 (UniProt: P07061)
A: 22 - 392 (UniProt: P07062)
A: 22 - 392 (UniProt: P07062)
| PDB | External Database | Details |
|---|---|---|
| Ala 112 | Ser 112 | engineered mutation |
| Asp 181 | Gly 181 | engineered mutation |
| Arg 190 | Ala 190 | SEE REMARK 999 |
| His 191 | Thr 191 | SEE REMARK 999 |
| Asn 266 | His 266 | engineered mutation |
| Tyr 370 | Asp 370 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 42905.6 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1398.4 | |
| All* | Total formula weight | 44303.9 |
