2ZM9

Structure of 6-Aminohexanoate-dimer Hydrolase, A61V/S112A/A124V/R187S/F264C/G291R/G338A/D370Y mutant (Hyb-S4M94) with Substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	6-aminohexanoate-dimer hydrolase	polymer	392	42927.7	1	UniProt (P07061) UniProt (P07062) Pfam (PF00144)	Flavobacterium sp.	Nylon oligomers-degrading enzyme EII, Nylon oligomers-degrading enzyme EII'
2	B, C, D (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3	E, F (A)	6-AMINOHEXANOIC ACID	non-polymer		131.2	2	Chemie (ACA)
4	G, H (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
5	I (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	J (A)	water	water		18.0	463	Chemie (HOH)

Sequence modifications

A: 1 - 21 (UniProt: P07061)
A: 22 - 392 (UniProt: P07062)

PDB	External Database	Details
Val 61	Ala 61	engineered mutation
Ala 112	Ser 112	engineered mutation
Val 124	Ala 124	engineered mutation
Ser 187	Arg 187	engineered mutation
Arg 190	Ala 190	SEE REMARK 999
His 191	Thr 191	SEE REMARK 999
Cys 264	Phe 264	engineered mutation
Arg 291	Gly 291	engineered mutation
Ala 338	Gly 338	engineered mutation
Tyr 370	Asp 370	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42927.7
Non-Polymers*	Number of molecules	8
	Total formula weight	1033.1
All*	Total formula weight	43960.8

*Water molecules are not included.