2ZIN
Crystal structure of the catalytic domain of pyrrolysyl-tRNA synthetase in complex with BocLys and an ATP analogue
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pyrrolysyl-tRNA synthetase | polymer | 291 | 33344.2 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | Methanosarcina mazei | Pyrrolysine--tRNA ligase, PylRS |
| 2 | B, C (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 4 | E (A) | N~6~-(tert-butoxycarbonyl)-L-lysine | non-polymer | 246.3 | 1 | Chemie (LBY) | |||
| 5 | F (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | G (A) | water | water | 18.0 | 217 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Met -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Ser -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Val -7 | - | expression tag |
| Pro -6 | - | expression tag |
| Arg -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Ser -3 | - | expression tag |
| His -2 | - | expression tag |
| Met -1 | - | expression tag |
| Gly 444 | Glu 444 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33344.2 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 863.2 | |
| All* | Total formula weight | 34207.3 |
