2ZAC

Crystal Structure of Family 7 Alginate Lyase A1-II' Y284F in Complex with Product (MMG)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alginate lyase	polymer	236	25777.5	1	UniProt (Q75WP3) Pfam (PF08787) In PDB	Sphingomonas sp.	Alginate lyase A1-II
2	B	beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid	branched		546.4	1
3	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	344	Chemie (HOH)

Sequence modifications

A: 81 - 308 (UniProt: Q75WP3)

PDB	External Database	Details
Phe 284	Tyr 284	engineered mutation
Leu 309	-	expression tag
Glu 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25777.5
Branched	Number of molecules	1
	Total formula weight	546.4
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	26416.0

*Water molecules are not included.