2ZAA
Crystal Structure of Family 7 Alginate Lyase A1-II' H191N/Y284F in Complex with Substrate (GGMG)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Alginate lyase | polymer | 236 | 25753.5 | 1 | UniProt (Q75WP3) Pfam (PF08787) In PDB | Sphingomonas sp. | Alginate lyase A1-II |
| 2 | B | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid | branched | 704.5 | 1 | In PDB GlyTouCan (G30171WO) | |||
| 3 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | water | water | 18.0 | 260 | Chemie (HOH) |
Sequence modifications
A: 81 - 308 (UniProt: Q75WP3)
| PDB | External Database | Details |
|---|---|---|
| Asn 191 | His 191 | engineered mutation |
| Phe 284 | Tyr 284 | engineered mutation |
| Leu 309 | - | expression tag |
| Glu 310 | - | expression tag |
| His 311 | - | expression tag |
| His 312 | - | expression tag |
| His 313 | - | expression tag |
| His 314 | - | expression tag |
| His 315 | - | expression tag |
| His 316 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25753.5 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 704.5 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 92.1 | |
| All* | Total formula weight | 26550.0 |
