2ZA9

Crystal Structure of Alginate lyase A1-II' N141C/N199C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alginate lyase	polymer	236	25771.6	1	UniProt (Q75WP3) Pfam (PF08787) In PDB	Sphingomonas sp.	Alginate lyase A1-II
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3		water	water		18.0	185	Chemie (HOH)

Sequence modifications

A: 81 - 308 (UniProt: Q75WP3)

PDB	External Database	Details
Cys 141	Asn 141	engineered mutation
Cys 199	Asn 199	engineered mutation
Leu 309	-	expression tag
Glu 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25771.6
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	25963.7

*Water molecules are not included.