2ZA4

Crystal Structural Analysis of Barnase-barstar Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Ribonuclease	polymer	110	12340.6	2	UniProt (P00648) Pfam (PF00545)	Bacillus amyloliquefaciens	Barnase, RNase Ba
2	B, D (B, D)	Barstar	polymer	90	10244.6	2	UniProt (P11540) Pfam (PF01337)	Bacillus amyloliquefaciens	Ribonuclease inhibitor
3	E, F (C, D)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	G, H, I, J (A, B, C, D)	water	water		18.0	448	Chemie (HOH)

Sequence modifications

A, C: 1 - 110 (UniProt: P00648)

PDB	External Database	Details
Ala 98	Lys 145	engineered mutation

B, D: 0 - 89 (UniProt: P11540)

PDB	External Database	Details
Ala 39	Asp 40	engineered mutation
Ala 40	Cys 41	engineered mutation
Ala 82	Cys 83	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45170.4
Non-Polymers*	Number of molecules	2
	Total formula weight	70.9
All*	Total formula weight	45241.3

*Water molecules are not included.