2Z6A
S-Adenosyl-L-methionine-Dependent Methyl Transfer: Observable Precatalytic Intermediates during DNA Cytosine Methylation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (C) | DNA (5'-D(*DGP*DAP*DTP*DAP*DGP*DCP*DGP*DCP*DTP*DAP*DTP*DC)-3') | polymer | 12 | 3662.4 | 1 | synthetic construct | ||
| 2 | B (D) | DNA (5'-D(*DTP*DGP*DAP*DTP*DAP*DGP*DCP*DGP*DCP*DTP*DAP*DTP*DC)-3') | polymer | 13 | 3966.6 | 1 | synthetic construct | ||
| 3 | C (A) | Modification methylase HhaI | polymer | 327 | 37010.1 | 1 | UniProt (P05102) Pfam (PF00145) | Haemophilus parahaemolyticus | HHAI METHYLTRANSFERASE, Cytosine-specific methyltransferase HhaI, M.HhaI |
| 4 | D (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) | |||
| 5 | E, F, G (C, D, A) | water | water | 18.0 | 55 | Chemie (HOH) |
Sequence modifications
A: 1 - 327 (UniProt: P05102)
| PDB | External Database | Details |
|---|---|---|
| Ala 81 | Cys 81 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 44639.1 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 384.4 | |
| All* | Total formula weight | 45023.6 |
