2Z4I

Crystal structure of the Cpx pathway activator NlpE from Escherichia coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Copper homeostasis protein cutF	polymer	233	26153.5	2	UniProt (P40710) Pfam (PF04170) Pfam (PF17185) In PDB	Escherichia coli	Lipoprotein nlpE
2	A	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
3	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4		water	water		18.0	24	Chemie (HOH)

Sequence modifications

A, B: 1 - 216 (UniProt: P40710)

PDB	External Database	Details
Ala 1	Cys 21	engineered mutation
Mse 217	-	expression tag
Ala 218	-	expression tag
Ser 219	-	expression tag
Mse 220	-	expression tag
Thr 221	-	expression tag
Gly 222	-	expression tag
Gly 223	-	expression tag
Gln 224	-	expression tag
Gln 225	-	expression tag
Mse 226	-	expression tag
Gly 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52306.9
Non-Polymers*	Number of molecules	2
	Total formula weight	378.4
All*	Total formula weight	52685.3

*Water molecules are not included.