2Z1J

Crystal structure of E.coli RNase HI surface charged mutant(Q4R/T40E/Q72H/Q76K/Q80E/T92K/Q105K/Q113R/Q115K/N143K/T145K)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ribonuclease HI	polymer	155	17794.5	1	UniProt (P0A7Y4) Pfam (PF00075) In PDB	Escherichia coli	RNase HI, Ribonuclease H, RNase H
2		water	water		18.0	43	Chemie (HOH)

Sequence modifications

A: 1 - 155 (UniProt: P0A7Y4)

PDB	External Database	Details
Arg 4	Gln 4	engineered mutation
Glu 40	Thr 40	engineered mutation
His 72	Gln 72	engineered mutation
Lys 76	Gln 76	engineered mutation
Glu 80	Gln 80	engineered mutation
Lys 92	Thr 92	engineered mutation
Lys 105	Gln 105	engineered mutation
Arg 113	Gln 113	engineered mutation
Lys 115	Gln 115	engineered mutation
Lys 143	Asn 143	engineered mutation
Lys 145	Thr 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	17794.5
All*	Total formula weight	17794.5

*Water molecules are not included.