2YXV

The deletion mutant of Multicopper Oxidase CueO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Blue copper oxidase cueO	polymer	446	48631.5	2	UniProt (P36649) Pfam (PF07732) Pfam (PF00394) Pfam (PF07731) In PDB	Escherichia coli	Copper efflux oxidase, Multicopper Oxidase CueO
2	A, B	COPPER (II) ION	non-polymer		63.5	4	Chemie (CU)
3	A	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)
4	A, B	CU-O-CU LINKAGE	non-polymer		143.1	2	Chemie (C2O)
5		water	water		18.0	395	Chemie (HOH)

Sequence modifications

A, B: 29 - 516 (UniProt: P36649)

PDB	External Database	Details
Gly 357	Pro 357	engineered mutation
-	Met 358	deletion
-	Leu 359	deletion
-	Asp 360	deletion
-	Met 361	deletion
-	Met 362	deletion
-	Gly 363	deletion
-	Met 364	deletion
-	Gln 365	deletion
-	Met 366	deletion
-	Leu 367	deletion
-	Met 368	deletion
-	Glu 369	deletion
-	Lys 370	deletion
-	Tyr 371	deletion
-	Gly 372	deletion
-	Asp 373	deletion
-	Gln 374	deletion
-	Ala 375	deletion
-	Met 376	deletion
-	Ala 377	deletion
-	Gly 378	deletion
-	Met 379	deletion
-	Asp 380	deletion
-	His 381	deletion
-	Ser 382	deletion
-	Gln 383	deletion
-	Met 384	deletion
-	Met 385	deletion
-	Gly 386	deletion
-	His 387	deletion
-	Met 388	deletion
-	Gly 389	deletion
-	His 390	deletion
-	Gly 391	deletion
-	Asn 392	deletion
-	Met 393	deletion
-	Asn 394	deletion
-	His 395	deletion
-	Met 396	deletion
-	Asn 397	deletion
-	His 398	deletion
-	Gly 399	deletion
-	Gly 400	deletion
-	Lys 401	deletion
-	Phe 402	deletion
-	Asp 403	deletion
-	Phe 404	deletion
-	His 405	deletion
Gly 406	His 406	engineered mutation
Gly 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag
His 521	-	expression tag
His 522	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97263.0
Non-Polymers*	Number of molecules	7
	Total formula weight	602.4
All*	Total formula weight	97865.4

*Water molecules are not included.