2YHW

High-resolution crystal structures of N-Acetylmannosamine kinase: Insights about substrate specificity, activity and inhibitor modelling.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	BIFUNCTIONAL UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE/N-ACETYLMANNOSAMINE KINASE	polymer	343	36620.0	1	UniProt (Q9Y223) Pfam (PF00480) In PDB	HOMO SAPIENS (HUMAN)	N-ACETYL MANNOSAMINE KINASE, MANAC KINASE, UDP-GLCNAC-2-EPIMERASE/MANAC KINASE
2	A	2-acetamido-2-deoxy-alpha-D-mannopyranose	non-polymer		221.2	1	Chemie (BM3)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	NONAETHYLENE GLYCOL	non-polymer		414.5	1	Chemie (2PE)
5	A	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
6	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
7	A	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
8	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
9	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
10		water	water		18.0	255	Chemie (HOH)

Sequence modifications

A: 406 - 720 (UniProt: Q9Y223)

PDB	External Database	Details
Met 378	-	expression tag
Gly 379	-	expression tag
Ser 380	-	expression tag
Ser 381	-	expression tag
His 382	-	expression tag
His 383	-	expression tag
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
Ser 388	-	expression tag
Ser 389	-	expression tag
Gly 390	-	expression tag
Leu 391	-	expression tag
Val 392	-	expression tag
Pro 393	-	expression tag
Arg 394	-	expression tag
Gly 395	-	expression tag
Ser 396	-	expression tag
His 397	-	expression tag
Met 398	-	expression tag
Glu 399	-	expression tag
Asn 400	-	expression tag
Leu 401	-	expression tag
Tyr 402	-	expression tag
Phe 403	-	expression tag
Gln 404	-	expression tag
Gly 405	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36620.0
Non-Polymers*	Number of molecules	12
	Total formula weight	1384.3
All*	Total formula weight	38004.3

*Water molecules are not included.