2YGY

Structure of wild type E. coli N-acetylneuraminic acid lyase in space group P21 crystal form II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-ACETYLNEURAMINATE LYASE	polymer	304	33584.4	4	UniProt (P0A6L4) Pfam (PF00701)	ESCHERICHIA COLI	N-ACETYLNEURAMINIC ACID LYASE, N-ACETYLNEURAMINATE PYRUVATE- LYASE, N-ACETYLNEURAMINIC ACID ALDOLASE, NALASE, SIALATE LYASE, SIALIC ACID ALDOLASE, SIALIC ACID LYASE
2	E, F, H, J (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
3	G, I (B, C)	PENTAETHYLENE GLYCOL	non-polymer		238.3	2	Chemie (1PE)
4	K, L, M, N (A, B, C, D)	water	water		18.0	653	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 297 (UniProt: P0A6L4)

PDB	External Database	Details
Met -6	-	expression tag
Glu -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134337.5
Non-Polymers*	Number of molecules	6
	Total formula weight	618.4
All*	Total formula weight	134955.8

*Water molecules are not included.