2YA3
STRUCTURE OF THE REGULATORY FRAGMENT OF MAMMALIAN AMPK IN COMPLEX WITH COUMARIN ADP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 5'-AMP-ACTIVATED PROTEIN KINASE CATALYTIC SUBUNIT ALPHA-1 | polymer | 173 | 19486.8 | 1 | UniProt (P54645) Pfam (PF16579) | RATTUS NORVEGICUS (NORWAY RAT) | AMPK SUBUNIT ALPHA-1 |
| 2 | B (B) | 5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-2 | polymer | 87 | 10040.8 | 1 | UniProt (O43741) Pfam (PF04739) | HOMO SAPIENS (HUMAN) | AMPK SUBUNIT BETA-2 |
| 3 | C (E) | 5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-1 | polymer | 330 | 37434.1 | 1 | UniProt (P80385) Pfam (PF00571) | RATTUS NORVEGICUS (NORWAY RAT) | AMPK SUBUNIT ALPHA-1, AMPK GAMMA1, AMPK SUBUNIT GAMMA-1, AMPKG |
| 4 | D, E (E) | 3'-(7-diethylaminocoumarin-3-carbonylamino)-3'-deoxy-ADP | non-polymer | 669.5 | 2 | Chemie (J7V) PubChem (50919258) | |||
| 5 | F (E) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (224) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (16397049) PubChem (16760266) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (46874558) PubChem (40459109) PubChem (46875232) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (12358354) PubChem (122197642) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 6 | G, H, I (A, B, E) | water | water | 18.0 | 142 | Chemie (HOH) |
Sequence modifications
A: 396 - 544 (UniProt: P54645)
B: 187 - 272 (UniProt: O43741)
| PDB | External Database | Details |
|---|---|---|
| Met 378 | - | expression tag |
| Ser 379 | - | expression tag |
| His 380 | - | expression tag |
| His 381 | - | expression tag |
| His 382 | - | expression tag |
| His 383 | - | expression tag |
| His 384 | - | expression tag |
| His 385 | - | expression tag |
| Ser 386 | - | expression tag |
| Gly 387 | - | expression tag |
| Leu 388 | - | expression tag |
| Val 389 | - | expression tag |
| Pro 390 | - | expression tag |
| Arg 391 | - | expression tag |
| Gly 392 | - | expression tag |
| Ser 393 | - | cloning artifact |
| Met 394 | - | cloning artifact |
| Ala 395 | - | cloning artifact |
| Asn 545 | - | cloning artifact |
| Ser 546 | - | cloning artifact |
| Cys 547 | - | cloning artifact |
| Thr 548 | - | cloning artifact |
| Val 549 | - | cloning artifact |
| Asn 550 | - | cloning artifact |
| PDB | External Database | Details |
|---|---|---|
| Met 186 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 66961.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1686.2 | |
| All* | Total formula weight | 68647.9 |
