2Y70

CRYSTALLOGRAPHIC STRUCTURE OF GM23, MUTANT G89D, AN EXAMPLE OF CATALYTIC MIGRATION FROM TIM TO THIAMIN PHOSPHATE SYNTHASE.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	TRIOSE-PHOSPHATE ISOMERASE	polymer	245	26376.1	4	UniProt (P04789) Pfam (PF00121)	TRYPANOSOMA BRUCEI BRUCEI	GM23, TIM
2	E, L, R, U (A, B, C, D)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	F, G, H, I, J... (A, B, C, D)	ACETATE ION	non-polymer		59.0	17	Chemie (ACT)
4	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	562	Chemie (HOH)

Sequence modifications

A, B, C, D: 21 - 265 (UniProt: P04789)

PDB	External Database	Details
Pro 63	Ser 43	engineered mutation
Ser 64	Thr 44	engineered mutation
Trp 65	Phe 45	engineered mutation
Tyr 66	Val 46	engineered mutation
Met 67	His 47	engineered mutation
Gly 68	-	insertion
Ala 69	-	insertion
Gln 70	Leu 48	engineered mutation
Gly 90	Ile 68	engineered mutation
Asn 91	Ala 69	engineered mutation
Ala 92	Lys 70	engineered mutation
Asp 93	Ser 71	engineered mutation
-	Gly 72	deletion
-	Ala 73	deletion
-	Phe 74	deletion
-	Thr 75	deletion
-	Gly 76	deletion
-	Glu 77	deletion
-	Val 78	deletion
Ala 94	Ser 79	engineered mutation
Ala 96	Pro 81	engineered mutation
Ser 97	Ile 82	engineered mutation
Ile 103	Val 88	engineered mutation
Ser 104	Asn 89	engineered mutation
Asp 109	Gly 94	engineered mutation
Ser 150	Glu 135	engineered mutation
Thr 163	Ala 148	engineered mutation
Val 213	Ile 198	engineered mutation
Arg 232	Lys 217	engineered mutation
Glu 265	Gln 250	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105504.3
Non-Polymers*	Number of molecules	21
	Total formula weight	1388.0
All*	Total formula weight	106892.3

*Water molecules are not included.