2Y6Z
Crystallographic structure of GM23 an example of Catalytic migration from TIM to thiamin phosphate synthase.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | TRIOSE-PHOSPHATE ISOMERASE | polymer | 256 | 27158.0 | 1 | UniProt (P04789) Pfam (PF00121) | TRYPANOSOMA BRUCEI BRUCEI | GM23, TIM |
| 2 | B (A) | THIAMIN PHOSPHATE | non-polymer | 345.3 | 1 | Chemie (TPS) | |||
| 3 | C (A) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 1 | Chemie (POP) | |||
| 4 | D (A) | water | water | 18.0 | 85 | Chemie (HOH) |
Sequence modifications
A: 21 - 265 (UniProt: P04789)
| PDB | External Database | Details |
|---|---|---|
| Ser 10 | - | expression tag |
| Ser 11 | - | expression tag |
| Ser 12 | - | expression tag |
| Gly 13 | - | expression tag |
| Leu 14 | - | expression tag |
| Val 15 | - | expression tag |
| Pro 16 | - | expression tag |
| Arg 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Ser 19 | - | expression tag |
| Ala 20 | - | expression tag |
| Gly 22 | Ser 2 | engineered mutation |
| Gly 37 | Ser 17 | engineered mutation |
| Gly 38 | Gln 18 | engineered mutation |
| Pro 63 | Ser 43 | engineered mutation |
| Ser 64 | Thr 44 | engineered mutation |
| Trp 65 | Phe 45 | engineered mutation |
| Tyr 66 | Val 46 | engineered mutation |
| Met 67 | His 47 | engineered mutation |
| Gly 68 | - | insertion |
| Ala 69 | - | insertion |
| Gln 70 | Leu 48 | engineered mutation |
| Gly 90 | Ile 68 | engineered mutation |
| Asn 91 | Ala 69 | engineered mutation |
| Ala 92 | Lys 70 | engineered mutation |
| Asp 93 | Ser 71 | engineered mutation |
| - | Gly 72 | deletion |
| - | Ala 73 | deletion |
| - | Phe 74 | deletion |
| - | Thr 75 | deletion |
| - | Gly 76 | deletion |
| - | Glu 77 | deletion |
| - | Val 78 | deletion |
| Ala 94 | Ser 79 | engineered mutation |
| Ala 96 | Pro 81 | engineered mutation |
| Ser 97 | Ile 82 | engineered mutation |
| Ile 103 | Val 88 | engineered mutation |
| Ser 104 | Asn 89 | engineered mutation |
| Ser 150 | Glu 135 | engineered mutation |
| Thr 163 | Ala 148 | engineered mutation |
| Val 213 | Ile 198 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27158.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 521.3 | |
| All* | Total formula weight | 27679.3 |
