2Y6L

Xylopentaose binding X-2 engineered mutated CBM4-2 Carbohydrate Binding Module from a Thermostable Rhodothermus marinus Xylanase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	XYLANASE	polymer	167	17905.7	1	UniProt (Q6V8M0) Pfam (PF02018) UniProt (by SIFTS) (Q7WTN6)	RHODOTHERMUS MARINUS	X-2 ENGINEERED VARIANT XYLANASE
2	B (B)	beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose	branched		678.6	1	In PDB GlyTouCan (G47101GE)
3	C, D (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	E (A)	water	water		18.0	227	Chemie (HOH)

Sequence modifications

A: 2 - 166 (UniProt: Q6V8M0)

PDB	External Database	Details
Met 1	-	SEE REMARK 999
Phe 69	Trp 68	engineered mutation
Asn 70	Asp 69	engineered mutation
Gln 72	Glu 71	engineered mutation
Leu 76	Phe 75	engineered mutation
Arg 91	Trp 90	engineered mutation
Leu 110	Phe 109	engineered mutation
Asp 111	Gln 110	engineered mutation
His 118	Glu 117	engineered mutation
Leu 167	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	17905.7
Branched	Number of molecules	1
	Total formula weight	678.6
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	18664.5

*Water molecules are not included.