2Y5Y

Crystal structure of LacY in complex with an affinity inactivator

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LACTOSE PERMEASE	polymer	423	47271.5	2	UniProt (P02920) Pfam (PF01306)	ESCHERICHIA COLI	LACTOSE-PROTON SYMPORT
2	C, E (A, B)	BARIUM ION	non-polymer		137.3	2	Chemie (BA) PubChem (104810)
3	D, F (A, B)	2-sulfanylethyl beta-D-galactopyranoside	non-polymer		240.3	2	Chemie (TGA) PubChem (11085880) PubChem (45039703) PubChem (44311146) PubChem (53320289) PubChem (54760216) PubChem (9942823) PubChem (58040650) PubChem (87172360) PubChem (87172580) PubChem (87172581) PubChem (87172648) PubChem (88561693) PubChem (88589250) PubChem (88589256) PubChem (88589258) PubChem (89197544) PubChem (89876976) PubChem (89876981) PubChem (93090115) PubChem (93090117) PubChem (93532289) PubChem (93532290) PubChem (117616279) PubChem (124193334) PubChem (124193335) PubChem (124193339) PubChem (124197115) PubChem (124197118) PubChem (124197119) PubChem (129018324) PubChem (129036040) PubChem (129036041) PubChem (129036055) PubChem (129849941) PubChem (132770116)

Sequence modifications

A, B: 1 - 417 (UniProt: P02920)

PDB	External Database	Details
Ser 117	Cys 117	engineered mutation
Cys 122	Ala 122	engineered mutation
Ala 148	Cys 148	engineered mutation
Val 154	Cys 154	engineered mutation
Met 176	Cys 176	engineered mutation
Ser 234	Cys 234	engineered mutation
Ser 333	Cys 333	engineered mutation
Ala 353	Cys 353	engineered mutation
Ala 355	Cys 355	engineered mutation
His 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	94543.0
Non-Polymers*	Number of molecules	4
	Total formula weight	755.2
All*	Total formula weight	95298.2

*Water molecules are not included.