2XXJ

Penta mutant of lactate dehydrogenase from Thermus thermophilus, ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L-LACTATE DEHYDROGENASE	polymer	310	32599.4	4	UniProt (Q5SJA1) Pfam (PF00056) Pfam (PF02866) In PDB	THERMUS THERMOPHILUS	L-LDH
2	A, C	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	B, D	OXAMIC ACID	non-polymer		89.0	2	Chemie (OXM)
4	B, D	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
5		water	water		18.0	643	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 310 (UniProt: Q5SJA1)

PDB	External Database	Details
Trp 58	Arg 58	engineered mutation
Ala 128	Arg 128	engineered mutation
Ala 260	Glu 260	engineered mutation
Ala 279	Glu 279	engineered mutation
Ala 292	Glu 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130397.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1697.1
All*	Total formula weight	132094.6

*Water molecules are not included.