2XXJ
Penta mutant of lactate dehydrogenase from Thermus thermophilus, ternary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | L-LACTATE DEHYDROGENASE | polymer | 310 | 32599.4 | 4 | UniProt (Q5SJA1) Pfam (PF00056) Pfam (PF02866) | THERMUS THERMOPHILUS | L-LDH |
| 2 | E, H (A, C) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 3 | F, I (B, D) | OXAMIC ACID | non-polymer | 89.0 | 2 | Chemie (OXM) | |||
| 4 | G, J (B, D) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
| 5 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 643 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 310 (UniProt: Q5SJA1)
| PDB | External Database | Details |
|---|---|---|
| Trp 58 | Arg 58 | engineered mutation |
| Ala 128 | Arg 128 | engineered mutation |
| Ala 260 | Glu 260 | engineered mutation |
| Ala 279 | Glu 279 | engineered mutation |
| Ala 292 | Glu 292 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 130397.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1697.1 | |
| All* | Total formula weight | 132094.6 |
