2XRZ

X-ray structure of archaeal class II CPD photolyase from Methanosarcina mazei in complex with intact CPD-lesion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DEOXYRIBODIPYRIMIDINE PHOTOLYASE	polymer	482	55123.5	2	UniProt (Q8PYK9) Pfam (PF00875)	METHANOSARCINA MAZEI	CLASS II CPD PHOTOLYASE
2	C, E (C, E)	CPD-COMPRISING OLIGONUCLEOTIDE	polymer	14	4520.0	2		SYNTHETIC CONSTRUCT
3	D, F (D, F)	COUNTERSTRAND-OLIGONUCLEOTIDE	polymer	14	4305.8	2		SYNTHETIC CONSTRUCT
4	G, P (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
5	H (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	I, J, K (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	L, M, N, O, Q... (A, B)	ACETATE ION	non-polymer		59.0	6	Chemie (ACT)
8	S, T, U, V, W... (A, B, C, D, E...)	water	water		18.0	635	Chemie (HOH)

Sequence modifications

A, B: 3 - 464 (UniProt: Q8PYK9)

PDB	External Database	Details
Met -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Pro -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
Thr 377	Met 377	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	127898.6
Non-Polymers*	Number of molecules	12
	Total formula weight	2297.7
All*	Total formula weight	130196.3

*Water molecules are not included.