2XLP
Joint-functions of protein residues and NADP(H) in oxygen-activation by flavin-containing monooxygenase: Asn78Ser mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | FLAVIN-CONTAINING MONOOXYGENASE | polymer | 461 | 53313.7 | 4 | UniProt (Q83XK4) | METHYLOPHAGA AMINISULFIDIVORANS | |
| 2 | E, I, O, R (A, B, C, D) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 4 | Chemie (FAD) | |||
| 3 | F, J, P, S (A, B, C, D) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) PubChem (5885) PubChem (46936685) PubChem (71717786) PubChem (4412) PubChem (90473931) PubChem (91972139) PubChem (145946263) PubChem (153311489) PubChem (174001641) PubChem (165382010) | |||
| 4 | G, K, Q, T (A, B, C, D) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 4 | Chemie (EPE) PubChem (23831) | |||
| 5 | H, L, M, N, U (A, B, D) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 5 | Chemie (PEG) PubChem (8117) | |||
| 6 | V, W, X, Y (A, B, C, D) | water | water | 18.0 | 96 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 6 - 461 (UniProt: Q83XK4)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Met 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Ser 5 | - | expression tag |
| Ser 78 | Asn 73 | engineered mutation |
| Ala 158 | Glu 153 | conflict |
| Ala 159 | Glu 154 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 213254.9 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 7599.6 | |
| All* | Total formula weight | 220854.6 |
