2XJU
X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae with mutation S227A in complex with calcium and a1,2-mannobiose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | FLOCCULATION PROTEIN FLO5 | polymer | 258 | 27716.5 | 1 | UniProt (P38894) Pfam (PF07691) In PDB | SACCHAROMYCES CEREVISIAE (BAKER'S YEAST) | FLOCCULIN-5 |
| 2 | B | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900111) GlyTouCan (G53402KW) | 2alpha-alpha-mannobiose | ||
| 3 | A | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | water | water | 18.0 | 434 | Chemie (HOH) |
Sequence modifications
A: 23 - 271 (UniProt: P38894)
| PDB | External Database | Details |
|---|---|---|
| Gly 14 | - | expression tag |
| Leu 15 | - | expression tag |
| Val 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Arg 18 | - | expression tag |
| Gly 19 | - | expression tag |
| Ser 20 | - | expression tag |
| His 21 | - | expression tag |
| Met 22 | - | expression tag |
| Ala 227 | Ser 227 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27716.5 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 253.7 | |
| All* | Total formula weight | 28312.5 |
