2XIW

Crystal structure of the Sac7d-derived IgG1-binder C3-C24S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-BINDING PROTEIN 7D	polymer	79	9168.0	2	UniProt (P13123) Pfam (PF02294)	SULFOLOBUS ACIDOCALDARIUS	NANOFITIN C3-C24S, 7 KDA DNA-BINDING PROTEIN D, SAC7D
2	C, D (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	E (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	F, G (A, B)	water	water		18.0	182	Chemie (HOH)

Sequence modifications

A, B: 2 - 66 (UniProt: P13123)

PDB	External Database	Details
Mse -10	-	expression tag
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Leu 67	-	expression tag
Asn 68	-	expression tag
Leu 7	Lys 7	engineered mutation
Leu 8	Tyr 8	engineered mutation
Asn 9	Lys 9	engineered mutation
Arg 21	Lys 21	engineered mutation
Asp 22	Lys 22	engineered mutation
Ser 24	Trp 24	engineered mutation
Gln 26	Val 26	engineered mutation
Asn 29	Met 29	engineered mutation
Lys 31	Ser 31	engineered mutation
Leu 33	Thr 33	engineered mutation
Asn 35	Asp 35	engineered mutation
Tyr 40	Thr 40	engineered mutation
Ala 42	Arg 42	engineered mutation
Asn 44	Ala 44	engineered mutation
Asp 46	Ser 46	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	18336.1
Non-Polymers*	Number of molecules	3
	Total formula weight	227.6
All*	Total formula weight	18563.7

*Water molecules are not included.