2XH7

Engineering the enolase active site pocket: Crystal structure of the D321A mutant of yeast enolase 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ENOLASE 1	polymer	443	47644.8	2	UniProt (P00924) Pfam (PF03952) Pfam (PF00113)	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	2-PHOSPHOGLYCERATE DEHYDRATASE 1,2-PHOSPHO-D-GLYCERATE HYDRO-LYASE 1
2	C, E, F, G, H... (A, B)	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
3	D, J (A, B)	2-PHOSPHOGLYCERIC ACID	non-polymer		186.1	2	Chemie (2PG)
4	O, P (A, B)	water	water		18.0	747	Chemie (HOH)

Sequence modifications

A, B: 1 - 436 (UniProt: P00924)

PDB	External Database	Details
Leu 437	-	expression tag
His 438	-	expression tag
His 439	-	expression tag
His 440	-	expression tag
His 441	-	expression tag
His 442	-	expression tag
His 443	-	expression tag
Ile 241	Val 242	conflict
Ala 321	Asp 322	engineered mutation
Ala 321	Asp 322	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95289.7
Non-Polymers*	Number of molecules	12
	Total formula weight	615.2
All*	Total formula weight	95904.9

*Water molecules are not included.