2XFG

Reassembly and co-crystallization of a family 9 processive endoglucanase from separately expressed GH9 and CBM3c modules

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ENDOGLUCANASE 1	polymer	466	52818.1	1	UniProt (Q02934) Pfam (PF00759)	CLOSTRIDIUM THERMOCELLUM	ENDOGLUCANASE I, EGI, ENDO-1,4-BETA-GLUCANASE, CELLULASE I
2	B (B)	ENDOGLUCANASE 1	polymer	176	19581.6	1	UniProt (Q02934) Pfam (PF00942)	CLOSTRIDIUM THERMOCELLUM	ENDOGLUCANASE I, EGI, ENDO-1,4-BETA-GLUCANASE, CELLULASE I
3	C, G (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	D, E, F (A)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
5	H, I (A, B)	water	water		18.0	982	Chemie (HOH)

Sequence modifications

A: -18 - 444 (UniProt: Q02934)

PDB	External Database	Details
Met -19	-	expression tag
Glu 445	-	expression tag
Leu 446	-	expression tag
Ser 27	Pro 99	conflict

B: 447 - 613 (UniProt: Q02934)

PDB	External Database	Details
Met 446	-	expression tag
Leu 614	-	expression tag
Glu 615	-	expression tag
His 616	-	expression tag
His 617	-	expression tag
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72399.7
Non-Polymers*	Number of molecules	5
	Total formula weight	186.5
All*	Total formula weight	72586.2

*Water molecules are not included.