2X72

CRYSTAL STRUCTURE OF THE CONSTITUTIVELY ACTIVE E113Q,D2C,D282C RHODOPSIN MUTANT WITH BOUND GALPHACT PEPTIDE.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RHODOPSIN	polymer	349	39033.6	1	UniProt (P02699) Pfam (PF10413) Pfam (PF00001) In PDB	BOS TAURUS (CATTLE)
2	B	GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-1	polymer	11	1265.5	1	UniProt (P04695) In PDB	BOS TAURUS	GACT PEPTIDE, TRANSDUCIN ALPHA-1 CHAIN
3	C	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
4	A	DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE	non-polymer		692.0	1	Chemie (PEF)
5	A	octyl beta-D-glucopyranoside	non-polymer		292.4	1	Chemie (BOG)
6	A	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
7	A	RETINAL	non-polymer		284.4	1	Chemie (RET)
8	A	2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE	non-polymer		648.9	1	Chemie (LPP)
9	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
10		water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 0 - 0 (PDB: 2X72)
A: 1 - 348 (UniProt: P02699)

PDB	External Database	Details
Cys 2	Asn 2	engineered mutation
Gln 113	Glu 113	engineered mutation
Cys 282	Asp 282	engineered mutation

B: 340 - 350 (UniProt: P04695)

PDB	External Database	Details
Leu 341	Lys 341	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40299.1
Branched	Number of molecules	1
	Total formula weight	910.8
Non-Polymers*	Number of molecules	7
	Total formula weight	2489.5
All*	Total formula weight	43699.5

*Water molecules are not included.