2X72
CRYSTAL STRUCTURE OF THE CONSTITUTIVELY ACTIVE E113Q,D2C,D282C RHODOPSIN MUTANT WITH BOUND GALPHACT PEPTIDE.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RHODOPSIN | polymer | 349 | 39033.6 | 1 | UniProt (P02699) Pfam (PF10413) Pfam (PF00001) | BOS TAURUS (CATTLE) | |
| 2 | B (B) | GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-1 | polymer | 11 | 1265.5 | 1 | UniProt (P04695) | BOS TAURUS | GACT PEPTIDE, TRANSDUCIN ALPHA-1 CHAIN |
| 3 | C (C) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 4 | D (A) | DI-PALMITOYL-3-SN-PHOSPHATIDYLETHANOLAMINE | non-polymer | 692.0 | 1 | Chemie (PEF) PubChem (445468) PubChem (65109) PubChem (15859389) | |||
| 5 | E (A) | octyl beta-D-glucopyranoside | non-polymer | 292.4 | 1 | Chemie (BOG) | |||
| 6 | F, G (A) | PALMITIC ACID | non-polymer | 256.4 | 2 | Chemie (PLM) | |||
| 7 | H (A) | RETINAL | non-polymer | 284.4 | 1 | Chemie (RET) PubChem (1070) PubChem (23254473) PubChem (177675058) PubChem (638015) PubChem (87082476) PubChem (87110449) PubChem (87110609) PubChem (88398773) PubChem (88407543) PubChem (5280490) PubChem (5353924) PubChem (5381765) PubChem (6436079) PubChem (6436082) PubChem (11426127) PubChem (12227253) PubChem (12227254) PubChem (12227256) PubChem (12227257) PubChem (12227258) PubChem (12227259) PubChem (12227269) PubChem (12227270) PubChem (12227271) PubChem (134688327) PubChem (140510408) PubChem (145826762) PubChem (169423542) PubChem (172878320) | |||
| 8 | I (A) | 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE | non-polymer | 648.9 | 1 | Chemie (LPP) PubChem (3099) PubChem (446066) PubChem (11061290) | |||
| 9 | J (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) PubChem (175) | |||
| 10 | K, L (A, B) | water | water | 18.0 | 18 | Chemie (HOH) |
Sequence modifications
A: 0 - 0 (PDB: 2X72)
A: 1 - 348 (UniProt: P02699)
B: 340 - 350 (UniProt: P04695)
A: 1 - 348 (UniProt: P02699)
| PDB | External Database | Details |
|---|---|---|
| Cys 2 | Asn 2 | engineered mutation |
| Gln 113 | Glu 113 | engineered mutation |
| Cys 282 | Asp 282 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 341 | Lys 341 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 40299.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 910.8 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 2489.5 | |
| All* | Total formula weight | 43699.5 |
