2X2Y
Cellulomonas fimi endo-beta-1,4-mannanase double mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | MAN26A | polymer | 475 | 50466.1 | 2 | UniProt (Q9XCV5) Pfam (PF02156) Pfam (PF17957) | CELLULOMONAS FIMI | BETA-1,4-MANNANASE |
| 2 | C, F, G, N (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 3 | D, E, H, I, J... (A, B) | FORMIC ACID | non-polymer | 46.0 | 8 | Chemie (FMT) | |||
| 4 | O, P (A, B) | water | water | 18.0 | 625 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 464 (UniProt: Q9XCV5)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Ala 465 | - | expression tag |
| Ala 466 | - | expression tag |
| Ala 467 | - | expression tag |
| Leu 468 | - | expression tag |
| Glu 469 | - | expression tag |
| His 470 | - | expression tag |
| His 471 | - | expression tag |
| His 472 | - | expression tag |
| His 473 | - | expression tag |
| His 474 | - | expression tag |
| His 475 | - | expression tag |
| Arg 323 | Ala 373 | engineered mutation |
| Ala 325 | Phe 375 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 100932.2 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 465.4 | |
| All* | Total formula weight | 101397.7 |
