2X2G

CRYSTALLOGRAPHIC BINDING STUDIES WITH AN ENGINEERED MONOMERIC VARIANT OF TRIOSEPHOSPHATE ISOMERASE

Replaces:  2X0M

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	TRIOSEPHOSPHATE ISOMERASE, GLYCOSOMAL	polymer	238	25659.3	2	UniProt (P04789) Pfam (PF00121)	TRYPANOSOMA BRUCEI BRUCEI	TRIOSEPHOSPHATE ISOMERASE, TIM
2	C (B)	3-PHOSPHOGLYCERIC ACID	non-polymer		186.1	1	Chemie (3PG)
3	D, E (A, B)	water	water		18.0	532	Chemie (HOH)

Sequence modifications

A, B: 15 - 72 (UniProt: P04789)

PDB	External Database	Details
Ser 15	Asn 15	engineered mutation
Pro 18	Gln 18	engineered mutation
Asp 19	Gln 19	engineered mutation
Gly 68	Ile 68	engineered mutation
Asn 69	Ala 69	engineered mutation
Ala 70	Lys 70	engineered mutation
Asp 71	Ser 71	engineered mutation
Ala 72	Gly 72	engineered mutation

A, B: 80 - 234 (UniProt: P04789)

PDB	External Database	Details
Ala 81	Pro 81	engineered mutation
Ser 82	Ile 82	engineered mutation
Trp 100	Ala 100	engineered mutation
Ala 233	Val 233	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51318.6
Non-Polymers*	Number of molecules	1
	Total formula weight	186.1
All*	Total formula weight	51504.6

*Water molecules are not included.