2X03

The X-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase Y253A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PUTATIVE SECRETED LYASE	polymer	765	82930.1	2	UniProt (O86516) Pfam (PF08124) Pfam (PF02278) Pfam (PF02884)	STREPTOMYCES COELICOLOR	PL8
2	C (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	D, E (A, B)	water	water		18.0	841	Chemie (HOH)

Sequence modifications

A, B: 22 - 765 (UniProt: O86516)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Ala 253	Tyr 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	165860.3
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	165884.6

*Water molecules are not included.