2WYS
High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | ENDO-1,4-BETA-XYLANASE Y | polymer | 540 | 60658.3 | 2 | UniProt (P51584) Pfam (PF02018) Pfam (PF00331) In PDB | CLOSTRIDIUM THERMOCELLUM | XYLANASE Y, ENDO-1,4-BETA-D-XYLANASE 10B |
2 | C | beta-D-xylopyranose-(1-2)-beta-D-xylopyranose | branched | 282.2 | 1 | In PDB GlyTouCan (G63975WQ) | |||
3 | D | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 414.4 | 1 | In PDB BIRD (PRD_900117) GlyTouCan (G87429QT) | 4beta-beta-xylotriose | ||
4 | A | beta-D-xylopyranose | non-polymer | 150.1 | 1 | Chemie (XYP) | |||
5 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
6 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
7 | water | water | 18.0 | 378 | Chemie (HOH) |
Sequence modifications
A, B: 33 - 551 (UniProt: P51584)
PDB | External Database | Details |
---|---|---|
Mse 12 | - | expression tag |
Gly 13 | - | expression tag |
Ser 14 | - | expression tag |
Ser 15 | - | expression tag |
His 16 | - | expression tag |
His 17 | - | expression tag |
His 18 | - | expression tag |
His 19 | - | expression tag |
His 20 | - | expression tag |
His 21 | - | expression tag |
Ser 22 | - | expression tag |
Ser 23 | - | expression tag |
Gly 24 | - | expression tag |
Leu 25 | - | expression tag |
Val 26 | - | expression tag |
Pro 27 | - | expression tag |
Arg 28 | - | expression tag |
Gly 29 | - | expression tag |
Ser 30 | - | expression tag |
His 31 | - | expression tag |
Mse 32 | - | expression tag |
Ala 337 | Glu 337 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 121316.5 | |
Branched | Number of molecules | 2 |
Total formula weight | 696.6 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 500.4 | |
All* | Total formula weight | 122513.5 |