2WS3

Crystal structure of the E. coli succinate:quinone oxidoreductase (SQR) SdhD Tyr83Phe mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (A, E, I)	SUCCINATE DEHYDROGENASE FLAVOPROTEIN SUBUNIT	polymer	588	64502.8	3	UniProt (P0AC41) Pfam (PF00890) Pfam (PF02910)	ESCHERICHIA COLI
2	B, F, J (B, F, J)	SUCCINATE DEHYDROGENASE IRON-SULFUR SUBUNIT	polymer	238	26800.9	3	UniProt (P07014) Pfam (PF13085) Pfam (PF13534)	ESCHERICHIA COLI
3	C, G, K (C, G, K)	SUCCINATE DEHYDROGENASE CYTOCHROME B-556 SUBUNIT	polymer	129	14313.1	3	UniProt (P69054) Pfam (PF01127)	ESCHERICHIA COLI	CYTOCHROME B-556
4	D, H, L (D, H, L)	SUCCINATE DEHYDROGENASE HYDROPHOBIC MEMBRANE ANCHOR SUBUNIT	polymer	115	12858.4	3	UniProt (P0AC44) Pfam (PF01127)	ESCHERICHIA COLI
5	CA, M, U (I, A, E)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	3	Chemie (FAD)
6	DA, N, V (I, A, E)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
7	EA, O, W (I, A, E)	MALATE LIKE INTERMEDIATE	non-polymer		132.1	3	Chemie (TEO)
8	FA, P, X (J, B, F)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	3	Chemie (FES)
9	GA, Q, Y (J, B, F)	IRON/SULFUR CLUSTER	non-polymer		351.6	3	Chemie (SF4)
10	HA, R, Z (J, B, F)	FE3-S4 CLUSTER	non-polymer		295.8	3	Chemie (F3S)
11	AA, IA, S (G, K, C)	2-METHYL-N-PHENYL-5,6-DIHYDRO-1,4-OXATHIINE-3-CARBOXAMIDE	non-polymer		235.3	3	Chemie (CBE)
12	BA, JA, T (G, K, C)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	3	Chemie (HEM)

Sequence modifications

D, H, L: 1 - 115 (UniProt: P0AC44)

PDB	External Database	Details
Phe 83	Tyr 83	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	355425.6
Non-Polymers*	Number of molecules	24
	Total formula weight	7847.0
All*	Total formula weight	363272.6

*Water molecules are not included.