2WNQ

Structure of the E192N mutant of E. coli N-acetylneuraminic acid lyase in space group P21

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-ACETYLNEURAMINATE LYASE	polymer	304	33569.4	4	UniProt (P0A6L4) Pfam (PF00701)	ESCHERICHIA COLI	N-ACETYLNEURAMINIC ACID LYASE, N-ACETYLNEURAMINATE PYRUVATE-LYASE, SIALIC ACID LYASE, SIALATE LYASE, SIALIC ACID ALDOLASE, NALASE
2	E, F, G, H (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
3	I, J, K, L (A, B, C, D)	water	water		18.0	316	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 297 (UniProt: P0A6L4)

PDB	External Database	Details
Met -6	-	expression tag
Glu -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Asn 192	Glu 192	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	134277.4
Non-Polymers*	Number of molecules	4
	Total formula weight	141.8
All*	Total formula weight	134419.2

*Water molecules are not included.