2WMK

Crystal structure of the catalytic module of a family 98 glycoside hydrolase from Streptococcus pneumoniae SP3-BS71 (Sp3GH98) in complex with the A-LewisY pentasaccharide blood group antigen.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	FUCOLECTIN-RELATED PROTEIN	polymer	606	69837.3	2	UniProt (A5LBQ0)	STREPTOCOCCUS PNEUMONIAE	GLYCOSIDE HYDROLASE
2	C, D (C, D)	alpha-L-fucopyranose-(1-2)-[2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)]beta-D-galactopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		878.8	2	In PDB BIRD (PRD_900127) GlyTouCan (G54826OZ)		Blood group A Lewis Y antigen, beta anomer
3	E, F (A, B)	water	water		18.0	974	Chemie (HOH)

Sequence modifications

A, B: 421 - 1005 (UniProt: A5LBQ0)

PDB	External Database	Details
Met 398	-	expression tag
Gly 399	-	expression tag
Ser 400	-	expression tag
Ser 401	-	expression tag
His 402	-	expression tag
His 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
Ser 408	-	expression tag
Ser 409	-	expression tag
Gly 410	-	expression tag
Leu 411	-	expression tag
Val 412	-	expression tag
Pro 413	-	expression tag
Arg 414	-	expression tag
Gly 415	-	expression tag
Ser 416	-	expression tag
His 417	-	expression tag
Met 418	-	expression tag
Ala 419	-	expression tag
Ser 420	-	expression tag
Ala 558	Glu 560	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	139674.6
Branched	Number of molecules	2
	Total formula weight	1757.6
All*	Total formula weight	141432.3

*Water molecules are not included.