2WKP

Structure of a photoactivatable Rac1 containing Lov2 Wildtype

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	NPH1-1, RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1	polymer	332	37603.0	1	UniProt (O49003) UniProt (P63000) Pfam (PF13426) Pfam (PF00071) In PDB	AVENA SATIVA (OAT)	RAC1_HUMAN, P21-RAC1, RAS-LIKE PROTEIN TC25, CELL MIGRATION-INDUCING GENE 5 PROTEIN
2	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
3	A	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A: 404 - 546 (UniProt: O49003)
A: 547 - 723 (UniProt: P63000)

PDB	External Database	Details
Met 392	-	expression tag
Arg 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
Gly 402	-	expression tag
Ser 403	-	expression tag
Leu 604	Gln 61	engineered mutation
His 634	Glu 91	engineered mutation
His 635	Asn 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	37603.0
Non-Polymers*	Number of molecules	4
	Total formula weight	1043.9
All*	Total formula weight	38646.9

*Water molecules are not included.