2WJZ
Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E (A, C, E) | IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE HISF | polymer | 253 | 27753.9 | 3 | UniProt (Q9X0C6) Pfam (PF00977) | THERMOTOGA MARITIMA | IGP SYNTHASE CYCLASE SUBUNIT, IGP SYNTHASE SUBUNIT HISF, IMGP SYNTHASE SUBUNIT HISF, IGPS SUBUNIT HISF |
| 2 | B, D, F (B, D, F) | IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH | polymer | 201 | 22980.4 | 3 | UniProt (Q9X0C8) Pfam (PF00117) Pfam (PF07685) | THERMOTOGA MARITIMA | IGP SYNTHASE GLUTAMINE AMIDOTRANSFERASE SUBUNIT, IGP SYNTHASE SUBUNIT HISH, IMGP SYNTHASE SUBUNIT HISH, TMHISH, IGPS SUBUNIT HISH, AMIDOTRANSFERASE HISH |
| 3 | G, H, I (A, C, E) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) | |||
| 4 | J, K, L, M, N... (A, B, C, D, E...) | water | water | 18.0 | 209 | Chemie (HOH) |
Sequence modifications
B, D, F: 1 - 201 (UniProt: Q9X0C8)
| PDB | External Database | Details |
|---|---|---|
| Ala 181 | Lys 181 | engineered mutation |
| Ala 138 | Tyr 138 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 152202.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 284.9 | |
| All* | Total formula weight | 152487.6 |
