2WHL
Understanding how diverse mannanases recognise heterogeneous substrates
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | BETA-MANNANASE | polymer | 294 | 32762.7 | 1 | UniProt (Q5YEX6) Pfam (PF00150) In PDB | BACILLUS AGARADHAERENS | BAMAN5 |
| 2 | B | beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-alpha-D-mannopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G98033RN) | |||
| 3 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 4 | water | water | 18.0 | 273 | Chemie (HOH) |
Sequence modifications
A: 4 - 299 (UniProt: Q5YEX6)
| PDB | External Database | Details |
|---|---|---|
| Ser 6 | Tyr 37 | engineered mutation |
| Arg 23 | Lys 54 | conflict |
| Ile 69 | Val 100 | conflict |
| Asn 100 | Asp 131 | conflict |
| Ser 135 | Ala 166 | conflict |
| Met 188 | Ile 219 | conflict |
| - | Gly 261 | deletion |
| - | Asp 262 | deletion |
| Thr 258 | Ala 289 | conflict |
| Ser 259 | Glu 290 | conflict |
| Gln 272 | Asn 303 | conflict |
| Asp 286 | Asn 317 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 32762.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 504.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 59.0 | |
| All* | Total formula weight | 33326.2 |
